cDNA Clone ubiquitin

• Plasmid: pET15-MGCSSG-Ubiquitin TAG64-6His
• Parent Plasmid: pET156P-1
• Expressed: MGCSSG-Ub TAG64-6His
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccATGGGTTGCTCTTCCGGCCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAATAGTCCACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGCATCACCATCACCATCACTAAGCGGCCGC

Amino Acid Sequence

MGSMGCSSGQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
AGKQLEDGRTLSDYNIQK*STLHLVLRLRGGHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29246
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: GCSSG sequence inserted between Met1 and Gln2. Clone also contains 3 extra residues (MGS) upstream of ubiquitin Met1; N-ter 6His deleted. C-terminal Hisx6 tag immediately follows GG (retained TAA stop codon).
• Low Temp: No