cDNA Clone ubiquitin

• Plasmid: pET15-UBIQUITIN-6HIS TAG34
• Parent Plasmid: pET156P-1
• Expressed: ubiquitin-His TAG34
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCT
CGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAG
ACAAGTAGGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAA
CAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTC
CACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGCATCACCATCACCATC
ACTAAGCGGCCGC

Amino Acid Sequence

MGSMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDK*GIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLRLRGGHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29180
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N-ter 6His deleted. C-terminal Hisx6 tag immediately follows GG (retain TAA stop codon). Clone has 3 extra residues (MGS) upstream of ubiquitin Met1
• Low Temp: No