cDNA Clone UBC13

• Plasmid: pET15 6HIS 3C UBC13 E55R + replacement of D94/K94 with LPPKGA S96R
• Parent Plasmid: pET15 6His 3C
• Expressed: 6His 3C UBC13 E55R + replacement of D93/K94 with LPPKGA S96R
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGCCGGGCTGCCCCGCAGGATCATCAAGGAAACCCAGCGTTTGCTGGCAGAACCAGTTCCTGGCATCAAAGCCGAACCAGATGAGAGCAACGCCCGTTATTTTCATGTGGTCATTGCTGGCCCTCAGGATTCCCCCTTTGAGGGAGGGACTTTTAAACTTAGACTATTCCTTCCAGAAGAATACCCAATGGCAGCCCCTAAAGTACGTTTCATGACCAAAATTTATCATCCTAATGTAGACAAGTTGGGAAGAATATGTTTAGATATTTTGAAATTACCTCCAAAGGGTGCTTGGCGCCCAGCACTGCAGATCCGCACAGTTCTGCTATCGATCCAGGCCTTGTTAAGTGCTCCCAATCCAGATGATCCATTAGCAAATGATGTAGCGGAGCAGTGGAAGACCAACGAAGCCCAAGCCATAGAAACAGCTAGAGCATGGACTAGGCTATATGCCATGAATAATATTTAAGAATTC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSAGLPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLRLFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKLPPKGAWRPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAMNNI*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU27544
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: S100R mutant is actually S96R of full length clone. S100R numbering comes from the insertion of extra amino acids as stated above
• Low Temp: No