cDNA Clone USP35

• Plasmid: pcDNA FRT TO Rhod ext-USP35 116-1018
• Parent Plasmid: pcDNA FRT TO
• Expressed: SRMNGTEGPNFYVPFSNKTVD-USP35 116-1018

Sequence of Insert

GGATCCAGCAGAATGAACGGGACCGAGGGCCCAAACTTCTACGTGCCTTTCTCCAACAAGACGGTGGACATGGCCGCCGATGAGGTGTTCGCCCTGCTGCGGCGGGAAGTGCTGAGAACCGTGTGCGAAAGACCCGGCCCTGCCGCCTGTGCTCAGGTGGCCAGACTGCTGGCCAGACACCCCAGATGCGTGCCCGACGGCCCCCACAGACTGCTGTTCTGTCAGCAGCTCGTGCGGTGCCTGGGCCGGTTCAGATGTCCTGCCGAAGGCGAAGAGGGCGCCGTGGAATTCCTGGAACAGGCCCAGCAGGTCAGCGGCCTGCTGGCACAGCTGTGGCGAGCCCAGCCTGCCGCCATCCTGCCCTGCCTGAAAGAACTGTTCGCCGTGATCAGCTGCGCCGAGGAAGAACCCCCTAGCAGCGCTCTGGCCAGCGTGGTGCAGCATCTGCCTCTGGAACTGATGGACGGCGTCGTGCGGAACCTGAGCAACGACGACAGCGTGACCGACAGCCAGATGCTGACCGCCATCAGCCGGATGATCGACTGGGTGTCCTGGCCCCTGGGCAAGAACATCGACAAGTGGATCATTGCCCTGCTGAAGGGCCTGGCCGCCGTGAAGAAGTTCAGCATCCTGATCGAGGTGTCCCTGACCAAGATCGAGAAGGTGTTCAGCAAGCTGCTGTACCCCATCGTGCGGGGAGCCGCCCTGAGCGTGCTGAAGTACATGCTGCTGACCTTCCAGCACAGCCACGAGGCCTTCCATCTGCTGCTGCCCCACATCCCCCCCATGGTGGCCAGCCTGGTCAAAGAGGACAGCAACAGCGGCACCAGCTGCCTGGAACAGCTGGCCGAGCTGGTGCACTGCATGGTGTTCCGGTTCCCCGGCTTCCCCGACCTGTACGAGCCCGTGATGGAAGCCATCAAGGACCTGCACGTGCCCAACGAGGACCGGATCAAGCAGCTGCTGGGCCAGGACGCCTGGACCAGCCAGAAGTCCGAGCTGGCCGGCTTCTACCCCAGGCTGATGGCCAAGAGCGACACCGGCAAGATCGGCCTGATCAACCTGGGCAATACCTGCTACGTGAACAGCATCCTGCAGGCCCTGTTCATGGCCAGCGACTTCCGGCACTGCGTGCTGCGGCTGACCGAGAACAACAGCCAGCCCCTGATGACCAAGCTGCAGTGGCTGTTCGGCTTTCTGGAACACTCCCAGAGGCCCGCTATCAGCCCCGAGAACTTCCTGAGCGCCAGCTGGACCCCCTGGTTCAGCCCTGGCACCCAGCAGGACTGCAGCGAGTACCTGAAGTATCTGCTGGACCGGCTGCACGAGGAAGAGAAAACCGGCACCCGGATCTGCCAGAAGCTGAAGCAGAGCAGCAGCCCCAGCCCCCCTGAGGAACCTCCTGCCCCAAGCAGCACCAGCGTGGAAAAGATGTTCGGCGGAAAGATCGTGACCAGAATCTGCTGCCTGTGCTGTCTGAACGTGTCCAGCCGGGAAGAGGCCTTCACCGACCTGAGCCTGGCCTTCCCACCCCCCGAGAGATGCAGAAGGCGGAGACTGGGCAGCGTGATGCGGCCCACCGAGGACATCACCGCCAGAGAGCTGCCTCCCCCAACAAGCGCCCAGGGACCTGGCAGAGTGGGCCCCAGACGGCAGCGGAAGCACTGCATCACCGAGGATACCCCCCCCACCAGCCTGTACATCGAGGGCCTGGACAGCAAAGAGGCTGGCGGCCAGAGCAGCCAGGAAGAGAGAATCGAGCGCGAGGAAGAGGGCAAAGAGGAACGCACCGAGAAAGAGGAAGTCGGCGAGGAAGAAGAGAGCACCAGAGGCGAGGGCGAGCGCGAAAAAGAGGAAGAAGTCGAAGAGGAAGAGGAAAAGGTCGAGAAAGAGACAGAGAAAGAGGCCGAGCAGGAAAAAGAAGAGGACTCCCTGGGAGCCGGCACCCACCCCGATGCCGCCATTCCTAGCGGCGAGAGAACCTGCGGCAGCGAGGGCAGCAGAAGCGTGCTGGACCTGGTCAACTACTTCCTGTCCCCCGAGAAGCTGACCGCCGAGAACCGGTACTACTGCGAGAGCTGCGCCAGCCTGCAGGACGCCGAGAAGGTGGTGGAACTGAGCCAGGGCCCCTGCTACCTGATCCTGACCCTGCTGAGATTCAGCTTCGACCTGCGGACCATGCGGCGGAGAAAGATTCTGGACGACGTGTCCATCCCCCTGCTGCTGAGGCTGCCTCTGGCTGGCGGAAGAGGCCAGGCCTACGACCTGTGCAGCGTGGTGGTGCACAGCGGCGTGTCCAGCGAGAGCGGCCACTACTACTGCTACGCCAGAGAGGGCGCTGCCAGACCAGCCGCCTCTCTGGGCACAGCCGATAGACCCGAGCCCGAGAACCAGTGGTATCTGTTCAACGACACCAGAGTGTCCTTCAGCAGCTTCGAGAGCGTGTCCAACGTGACCTCATTCTTCCCAAAGGACACCGCCTACGTGCTGTTCTACCGGCAGCGGCCTAGAGAGGGCCCTGAGGCCGAACTGGGCAGCTCCAGAGTGCGGACCGAGCCCACCCTGCACAAGGACCTGATGGAAGCTATCTCCAAGGACAACATCCTGTATCTGCAGGAACAGGAAAAAGAGGCCAGAAGCAGAGCCGCCTACATCAGCGCCCTGCCCACCAGCCCTCACTGGGGCAGAGGCTTCGACGAGGACAAGGATGAGGACGAGGGCAGCCCTGGCGGCTGCAACCCTGCTGGCGGCAATGGCGGCGACTTCCACCGGCTGGTGTTCTGAGCGGCCGC

Amino Acid Sequence

SRMNGTEGPNFYVPFSNKTVDMAADEVFALLRREVLRTVCERPGPAACAQVARLLARHPRCVPDGPHRLLFCQQLVRCLGRFRCPAEGEEGAVEFLEQAQQVSGLLAQLWRAQPAAILPCLKELFAVISCAEEEPPSSALASVVQHLPLELMDGVVRNLSNDDSVTDSQMLTAISRMIDWVSWPLGKNIDKWIIALLKGLAAVKKFSILIEVSLTKIEKVFSKLLYPIVRGAALSVLKYMLLTFQHSHEAFHLLLPHIPPMVASLVKEDSNSGTSCLEQLAELVHCMVFRFPGFPDLYEPVMEAIKDLHVPNEDRIKQLLGQDAWTSQKSELAGFYPRLMAKSDTGKIGLINLGNTCYVNSILQALFMASDFRHCVLRLTENNSQPLMTKLQWLFGFLEHSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEKTGTRICQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVSSREEAFTDLSLAFPPPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGRVGPRRQRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEERTEKEEVGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLGAGTHPDAAIPSGERTCGSEGSRSVLDLVNYFLSPEKLTAENRYYCESCASLQDAEKVVELSQGPCYLILTLLRFSFDLRTMRRRKILDDVSIPLLLRLPLAGGRGQAYDLCSVVVHSGVSSESGHYYCYAREGAARPAASLGTADRPEPENQWYLFNDTRVSFSSFESVSNVTSFFPKDTAYVLFYRQRPREGPEAELGSSRVRTEPTLHKDLMEAISKDNILYLQEQEKEARSRAAYISALPTSPHWGRGFDEDKDEDEGSPGGCNPAGGNGGDFHRLVF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU55847
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please clone USP35 116-1018 with an N-terminal SRMNGTEGPNFYVPFSNKTVD tag into pcDNA5 FRT/TO hygro. Thank you.
• Low Temp: No