cDNA Clone USP49

• Plasmid: pcDNA5D FRT/TO BirA R118G-USP49 C262A
• Parent Plasmid: pcDNA FRT TO BirA-MCS-HA
• Expressed: BirA R118G-USP49 C62A
• Tag: BirA
• Species: Human

Sequence of Insert

ATGAAGGACAACACCGTGCCCCTGAAGCTGATCGCCCTGCTGGCCAACGG
CGAGTTCCACTCTGGCGAGCAGCTGGGAGAGACCCTGGGAATGAGCAGAG
CCGCCATCAACAAGCACATCCAGACACTGAGAGACTGGGGAGTGGACGTG
TTCACCGTGCCTGGCAAGGGCTACAGCCTGCCTGAGCCTATCCAGCTGCT
GAACGCCAAGCAGATCCTGGGACAGCTGGATGGCGGAAGCGTGGCCGTGC
TGCCTGTGATCGACTCCACCAATCAGTACCTGCTGGACAGAATCGGAGAG
CTGAAGTCCGGCGACGCCTGCATCGCCGAGTACCAGCAGGCTGGCAGAGG
AGGCAGAGGACGGAAGTGGTTCAGCCCATTCGGAGCCAACCTGTACCTGT
CCATGTTCTGGAGACTGGAGCAGGGACCTGCTGCTGCCATCGGACTGAGT
CTGGTGATCGGAATCGTGATGGCCGAGGTGCTGAGAAAGCTGGGAGCCGA
CAAGGTGAGAGTGAAGTGGCCTAATGACCTGTACCTCCAGGACCGCAAGC
TGGCTGGCATCCTGGTGGAGCTGACAGGCAAGACAGGCGATGCCGCTCAG
ATCGTGATCGGAGCCGGAATCAACATGGCCATGAGAAGAGTGGAGGAGAG
CGTGGTGAACCAGGGCTGGATCACCCTGCAGGAGGCTGGCATCAACCTGG
ACCGGAACACCCTGGCCGCCATGCTGATCAGAGAGCTGAGAGCCGCTCTG
GAGCTGTTCGAGCAGGAGGGACTGGCTCCTTACCTGAGCAGATGGGAGAA
GCTGGACAACTTCATCAACAGACCTGTGAAGCTGATCATCGGCGACAAGG
AAATCTTCGGCATCTCCAGAGGAATCGACAAGCAGGGAGCTCTGCTGCTG
GAGCAGGACGGAATCATCAAGCCCTGGATGGGCGGAGAAATCTCCCTGAG
AAGCGCAGAGAAGGGATCTATGGATAGATGCAAACATGTAGGGCGGTTAC
GGCTCGCCCAGGACCACTCCATCCTGAACCCTCAGAAGTGGTGCTGCTTA
GAGTGTGCCACCACCGAGTCCGTGTGGGCCTGCCTCAAGTGCTCCCACGT
GGCCTGCGGCCGCTATATTGAGGACCACGCCCTGAAACACTTTGAGGAGA
CGGGACACCCGCTAGCCATGGAAGTCCGGGATCTCTACGTGTTCTGTTAC
CTGTGCAAGGACTACGTGCTCAATGATAACCCAGAGGGGGACCTGAAGCT
GCTAAGAAGCTCCCTCCTGGCGGTCCGGGGCCAGAAACAGGACACGCCGG
TGAGACGTGGGCGGACGCTGCGGTCCATGGCTTCGGGTGAGGACGTGGTC
CTGCCGCAGCGCGCTCCTCAGGGACAGCCGCAGATGCTCACGGCTCTGTG
GTACCGGCGTCAGCGCCTGCTGGCCAGGACGCTGCGGCTGTGGTTCGAGA
AGAGCTCCCGGGGCCAGGCGAAGCTGGAGCAGCGGCGGCAGGAGGAGGCC
CTGGAGCGCAAGAAGGAGGAGGCGCGGAGGCGGCGGCGCGAGGTGAAACG
GCGGCTGCTGGAGGAGCTGGCCAGCACCCCTCCGCGCAAGAGTGCACGGC
TGCTCCTGCACACGCCCCGCGACGCGGGCCCGGCTGCCTCGCGCCCCGCC
GCCCTCCCTACCTCACGCAGAGTGCCCGCCGCCACACTCAAGCTGCGTCG
CCAGCCGGCCATGGCCCCAGGCGTCACGGGCCTGCGCAACCTGGGCAACA
CCgcCTACATGAACTCCATCCTCCAGGTGCTCAGCCACCTCCAGAAGTTC
CGAGAATGTTTCCTCAACCTTGACCCTTCCAAAACGGAACATCTGTTTCC
CAAAGCCACCAACGGGAAGACTCAGCTTTCTGGCAAGCCAACCAACAGCT
CGGCCACGGAGCTGTCCTTGAGAAATGACAGGGCCGAGGCATGCGAGCGG
GAGGGCTTCTGCTGGAACGGCAGGGCCTCCATTAGTCGGAGTCTGGAGCT
CATCCAGAACAAGGAGCCGAGTTCAAAGCACATTTCCCTCTGCCGTGAAC
TGCACACCCTCTTCCGAGTCATGTGGTCCGGGAAGTGGGCCCTAGTGTCG
CCCTTCGCCATGCTGCACTCAGTGTGGAGCCTGATCCCTGCCTTCCGCGG
CTACGACCAACAGGACGCGCAGGAATTTCTCTGCGAGCTGCTGCACAAGG
TGCAGCAGGAACTCGAGTCTGAGGGCACCACACGCCGGATCCTCATCCCC
TTCTCCCAGAGGAAGCTCACCAAACAGGTCTTAAAGGTGGTGAATACCAT
ATTTCATGGGCAGCTGCTCAGTCAGGTCACATGTATATCATGCAATTACA
AATCCAATACCATTGAGCCCTTTTGGGACCTATCCCTGGAATTCCCTGAA
CGCTATCACTGCATAGAAAAGGGGTTTGTCCCTTTGAATCAAACAGAGTG
CTTGCTCACTGAGATGCTGGCCAAATTCACAGAGACAGAGGCCCTGGAAG
GGAGAATCTACGCTTGTGACCAGTGTAACAGCAAACGACGAAAATCCAAT
CCCAAACCCCTTGTTCTGAGTGAAGCTAGAAAGCAGTTAATGATCTACAG
ACTACCTCAGGTTCTCCGGCTGCACCTTAAAAGATTCAGGTGGTCTGGCC
GTAATCATCGAGAGAAGATTGGGGTCCATGTCGTCTTTGACCAGGTATTA
ACCATGGAACCTTACTGCTGCAGGGACATGCTCTCCTCTCTTGACAAAGA
GACCTTTGCCTATGATCTCTCCGCAGTGGTCATGCATCACGGGAAAGGGT
TTGGCTCAGGACACTACACAGCCTATTGCTACAACACAGAGGGAGGTGCG
TGCGCTTTACTCTGTGGGGTGGGGGACACGGAAAGGGGTTGAGATCCCCC
GGGGAATTCGCTAGCCTCGAGAAGCTTGCGGCCGCTTACCCATACGATGT
GCCAGATTACGCCTAA

Amino Acid Sequence

MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDV
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSMDRCKHVGRLRLAQDHSILNPQKWCCL
ECATTESVWACLKCSHVACGRYIEDHALKHFEETGHPLAMEVRDLYVFCY
LCKDYVLNDNPEGDLKLLRSSLLAVRGQKQDTPVRRGRTLRSMASGEDVV
LPQRAPQGQPQMLTALWYRRQRLLARTLRLWFEKSSRGQAKLEQRRQEEA
LERKKEEARRRRREVKRRLLEELASTPPRKSARLLLHTPRDAGPAASRPA
ALPTSRRVPAATLKLRRQPAMAPGVTGLRNLGNTAYMNSILQVLSHLQKF
RECFLNLDPSKTEHLFPKATNGKTQLSGKPTNSSATELSLRNDRAEACER
EGFCWNGRASISRSLELIQNKEPSSKHISLCRELHTLFRVMWSGKWALVS
PFAMLHSVWSLIPAFRGYDQQDAQEFLCELLHKVQQELESEGTTRRILIP
FSQRKLTKQVLKVVNTIFHGQLLSQVTCISCNYKSNTIEPFWDLSLEFPE
RYHCIEKGFVPLNQTECLLTEMLAKFTETEALEGRIYACDQCNSKRRKSN
PKPLVLSEARKQLMIYRLPQVLRLHLKRFRWSGRNHREKIGVHVVFDQVL
TMEPYCCRDMLSSLDKETFAYDLSAVVMHHGKGFGSGHYTAYCYNTEGGA
CALLCGVGDTERG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28136
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 49108. Mutagenesis required to remove STOP at end of USP49 and restore frame with C-terminal HA tag