cDNA Clone WIPI1

• Synonyms: ATG18
• Plasmid: pGEX C3 HALO thrombin-WIPI1
• Parent Plasmid: pGEX C3 HALO thrombin
• Expressed: GST-c3-HALO-thr-WIPI1
• Genbank: NP_060453.3
• Tag: HALO
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCCGGAATTCCGGATGGAGGCCGAGGCCGCGGACGCTCCCCCGGGCGGGGTTGAGTCGGCGCTCAGCTGCTTCTCTTTCAACCAGGACTGCACAT
CCCTAGCAATTGGAACTAAAGCCGGGTATAAGCTGTTTTCTCTGAGTTCTGTGGAGCAGCTGGATCAAGTCCACGGAAGCAATGAAATCCCGGACGTCTA
CATCGTGGAGCGCCTCTTCTCCAGCAGCCTGGTGGTGGTAGTCAGTCACACAAAACCACGGCAGATGAACGTGTATCACTTCAAGAAAGGCACAGAGATC
TGTAATTACAGCTACTCCAGCAACATCTTGTCCATAAGGCTGAACCGGCAAAGGCTGCTGGTTTGCCTAGAAGAGTCCATTTATATTCACAACATTAAAG
ACATGAAGCTGTTGAAGACCCTCCTGGATATTCCTGCAAACCCAACAGGTCTATGTGCTCTCTCTATCAACCATTCCAATTCTTACCTGGCCTATCCTGG
AAGCCTGACTTCAGGGGAGATTGTGCTTTATGATGGAAACTCCCTGAAAACAGTCTGCACTATTGCTGCCCATGAGGGAACACTAGCTGCCATCACCTTC
AATGCCTCAGGCTCCAAACTAGCAAGTGCGTCTGAAAAAGGCACAGTCATCCGGGTGTTCTCTGTCCCTGATGGGCAAAAGCTCTATGAGTTCCGGAGAG
GGATGAAAAGGTATGTGACAATCAGCTCTCTAGTGTTCAGTATGGATTCACAATTCCTCTGCGCCTCCAGTAACACCGAGACGGTACACATCTTCAAGCT
GGAACAGGTCACCAACAGTCGACCAGAAGAGCCTTCGACCTGGAGTGGCTACATGGGAAAGATGTTTATGGCTGCTACCAACTACCTCCCTACCCAGGTG
TCAGACATGATGCATCAGGACAGGGCTTTTGCCACTGCACGCTTGAACTTCTCCGGACAGAGGAACATCTGTACCCTCTCAACGATCCAGAAGTTGCCAC
GGCTGCTAGTTGCGTCATCCAGTGGACACCTTTATATGTACAATTTGGATCCTCAGGATGGAGGAGAGTGTGTCTTAATCAAAACCCACAGCTTGCTTGG
CTCAGGAACAACAGAAGAGAATAAAGAAAATGACCTCAGACCTTCCTTACCTCAGTCTTATGCAGCGACCGTAGCCAGACCAAGTGCATCTTCAGCCTCC
ACGGTGCCAGGTTATTCTGAGGACGGCGGGGCGCTGCGAGGAGAAGTTATTCCTGAACATGAGTTTGCGACGGGACCAGTGTGTCTTGATGATGAGAATG
AGTTTCCTCCTATAATCTTGTGCCGTGGAAATCAGAAGGGCAAAACGAAGCAGTCATGA

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSPEFRMEAEAADAPP
GGVESALSCFSFNQDCTSLAIGTKAGYKLFSLSSVEQLDQVHGSNEIPDV
YIVERLFSSSLVVVVSHTKPRQMNVYHFKKGTEICNYSYSSNILSIRLNR
QRLLVCLEESIYIHNIKDMKLLKTLLDIPANPTGLCALSINHSNSYLAYP
GSLTSGEIVLYDGNSLKTVCTIAAHEGTLAAITFNASGSKLASASEKGTV
IRVFSVPDGQKLYEFRRGMKRYVTISSLVFSMDSQFLCASSNTETVHIFK
LEQVTNSRPEEPSTWSGYMGKMFMAATNYLPTQVSDMMHQDRAFATARLN
FSGQRNICTLSTIQKLPRLLVASSSGHLYMYNLDPQDGGECVLIKTHSLL
GSGTTEENKENDLRPSLPQSYAATVARPSASSASTVPGYSEDGGALRGEV
IPEHEFATGPVCLDDENEFPPIILCRGNQKGKTKQS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28032
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee