cDNA Clone UBC12

• Plasmid: pCDF-PylT-6HIS(TAG3)-Ubc12
• Parent Plasmid: pCDF-PylT
• Expressed: 6HIS(TAG3)-Ubc12
• Genbank: P691081
• Tag: 6His
• Species: Human

Sequence of Insert

CCATGGGCTAGAGCCATCATCATCATCATCACAGCAGCGGCCTGGAAGTT
CTGTTCCAGGGGCCCGGATCCCCGGAATTCCCGGGGGTCGACTCGAAAGC
GGCCGCGATGATCAAGCTGTTCTCGCTGAAGCAGCAGAAGAAGGAGGAGG
AGTCGGCGGGCGGCACCAAGGGCAGCAGCAAGAAGGCGTCGGCGGCGCAG
CTGCGGATCCAGAAGGACATAAACGAGCTGAACCTGCCCAAGACGTGTGA
TATCAGCTTCTCAGATCCAGACGACCTCCTCAACTTCAAGCTGGTCATCT
GTCCTGATGAGGGCTTCTACAAGAGTGGGAAGTTTGTGTTCAGTTTTAAG
GTGGGCCAGGGTTACCCGCATGATCCCCCCAAGGTGAAGTGTGAGACAAT
GGTCTATCACCCCAACATTGACCTCGAaGGCAACGTCTGCCTCAACATCC
TCAGAGAGGACTGGAAGCCAGTCCTTACGATAAACTCCATAATTTATGGC
CTGCAGTATCTCTTCTTGGAGCCCAACCCCGAGGACCCACTGAACAAGGA
GGCCGCAGAGGTCCTGCAGAACAACCGGCGGCTGTTTGAGCAGAACGTGC
AGCGCTCCATGCGGGGTGGCTACATCGGCTCCACCTACTTTGAGCGCTGC
CTGAAATAACTCGAG

Amino Acid Sequence

MG*SHHHHHHSSGLEVLFQGPGSPEFPGVDSKAAAMIKLFSLKQQKKEEE
SAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVIC
PDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNIL
REDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQ
RSMRGGYIGSTYFERCLK*

• Vector Type: Bacterial
• Antibiotic: Spectinomycin
• DU Number: DU24657
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: internal Xho1 at nt427 killed