Synonyms
DA41, DSK2, UBQN, XDRP1, PLIC-1, FLJ90054
Plasmid
pGEX-C3-HALO_Thr-UBQLN1 S555N
Parent Plasmid
pGEX6P Halo Thr
Expressed
GST-HALO-UBQLN1 S555N
Genbank
Gene 29979 Isoform 1 = NM_013438.4 (NP_038466.2)
Tag
GST
HALO
Species
Human
Sequence of Insert
View Sequence
ggatccATGGCCGAGAGTGGTGAAAGCGGCGGTCCTCCGGGCTCCCAGGA
TAGCGCCGCCGGAGCCGAAGGTGCTGGCGCCCCCGCGGCCGCTGCCTCCG
CGGAGCCCAAAATCATGAAAGTCACCGTGAAGACCCCGAAGGAAAAGGAG
GAATTCGCCGTGCCCGAGAATAGCTCCGTCCAGCAGTTTAAGGAAGAAAT
CTCTAAACGTTTTAAATCACATACTGACCAACTTGTGTTGATATTTGCTG
GAAAAATTTTGAAAGATCAAGATACCTTGAGTCAGCATGGAATTCATGAT
GGACTTACTGTTCACCTTGTCATTAAAACACAAAACAGGCCTCAGGATCA
TTCAGCTCAGCAAACAAATACAGCTGGAAGCAATGTTACTACATCATCAA
CTCCTAATAGTAACTCTACATCTGGTTCTGCTACTAGCAACCCTTTTGGT
TTAGGTGGCCTTGGGGGACTTGCAGGTCTGAGTAGCTTGGGTTTGAATAC
TACCAACTTCTCTGAACTACAGAGTCAGATGCAGCGACAACTTTTGTCTA
ACCCTGAAATGATGGTCCAGATCATGGAAAATCCCTTTGTTCAGAGCATG
CTCTCAAATCCTGACCTGATGAGACAGTTAATTATGGCCAATCCACAAAT
GCAGCAGTTGATACAGAGAAATCCAGAAATTAGTCATATGTTGAATAATC
CAGATATAATGAGACAAACGTTGGAACTTGCCAGGAATCCAGCAATGATG
CAGGAGATGATGAGGAACCAGGACCGAGCTTTGAGCAACCTAGAAAGCAT
CCCAGGGGGATATAATGCTTTAAGGCGCATGTACACAGATATTCAGGAAC
CAATGCTGAGTGCTGCACAAGAGCAGTTTGGTGGTAATCCATTTGCTTCC
TTGGTGAGCAATACATCCTCTGGTGAAGGTAGTCAACCTTCCCGTACAGA
AAATAGAGATCCACTACCCAATCCATGGGCTCCACAGACTTCCCAGAGTT
CATCAGCTTCCAGCGGCACTGCCAGCACTGTGGGTGGCACTACTGGTAGT
ACTGCCAGTGGCACTTCTGGGCAGAGTACTACTGCGCCAAATTTGGTGCC
TGGAGTAGGAGCTAGTATGTTCAACACACCAGGAATGCAGAGCTTGTTGC
AACAAATAACTGAAAACCCACAACTGATGCAAAACATGTTGTCTGCCCCC
TACATGAGAAGCATGATGCAGTCACTgAGCCAGAATCCTGACCTTGCTGC
ACAGATGATGCTGAATAATCCCCTATTTGCTGGAAATCCTCAGCTTCAAG
AACAAATGAGACAACAGCTCCCAACTTTCCTCCAACAAATGCAGAATCCT
GATACACTATCAGCAATGTCAAACCCTAGAGCAATGCAGGCCTTGTTACA
GATTCAGCAGGGTTTACAGACATTAGCAACGGAAGCCCCGGGCCTCATCC
CAGGGTTTACTCCTGGCTTGGGGGCATTAGGAAGCACTGGAGGCTCTTCG
GGAACTAATGGATCTAACGCCACACCTAGTGAAAACACAAGTCCCACAGC
AGGAACCACTGAACCTGGACATCAGCAGTTTATTCAGCAGATGCTGCAGG
CTCTTGCTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCAGATTTCAG
CAACAACTGGAACAACTCAaTGCAATGGGATTTTTGAACCGTGAAGCAAA
CTTGCAAGCTCTAATAGCAACAGGAGGTGATATCAATGCAGCTATTGAAA
GGTTACTGGGCTCCCAGCCATCATAGagatct
TAGCGCCGCCGGAGCCGAAGGTGCTGGCGCCCCCGCGGCCGCTGCCTCCG
CGGAGCCCAAAATCATGAAAGTCACCGTGAAGACCCCGAAGGAAAAGGAG
GAATTCGCCGTGCCCGAGAATAGCTCCGTCCAGCAGTTTAAGGAAGAAAT
CTCTAAACGTTTTAAATCACATACTGACCAACTTGTGTTGATATTTGCTG
GAAAAATTTTGAAAGATCAAGATACCTTGAGTCAGCATGGAATTCATGAT
GGACTTACTGTTCACCTTGTCATTAAAACACAAAACAGGCCTCAGGATCA
TTCAGCTCAGCAAACAAATACAGCTGGAAGCAATGTTACTACATCATCAA
CTCCTAATAGTAACTCTACATCTGGTTCTGCTACTAGCAACCCTTTTGGT
TTAGGTGGCCTTGGGGGACTTGCAGGTCTGAGTAGCTTGGGTTTGAATAC
TACCAACTTCTCTGAACTACAGAGTCAGATGCAGCGACAACTTTTGTCTA
ACCCTGAAATGATGGTCCAGATCATGGAAAATCCCTTTGTTCAGAGCATG
CTCTCAAATCCTGACCTGATGAGACAGTTAATTATGGCCAATCCACAAAT
GCAGCAGTTGATACAGAGAAATCCAGAAATTAGTCATATGTTGAATAATC
CAGATATAATGAGACAAACGTTGGAACTTGCCAGGAATCCAGCAATGATG
CAGGAGATGATGAGGAACCAGGACCGAGCTTTGAGCAACCTAGAAAGCAT
CCCAGGGGGATATAATGCTTTAAGGCGCATGTACACAGATATTCAGGAAC
CAATGCTGAGTGCTGCACAAGAGCAGTTTGGTGGTAATCCATTTGCTTCC
TTGGTGAGCAATACATCCTCTGGTGAAGGTAGTCAACCTTCCCGTACAGA
AAATAGAGATCCACTACCCAATCCATGGGCTCCACAGACTTCCCAGAGTT
CATCAGCTTCCAGCGGCACTGCCAGCACTGTGGGTGGCACTACTGGTAGT
ACTGCCAGTGGCACTTCTGGGCAGAGTACTACTGCGCCAAATTTGGTGCC
TGGAGTAGGAGCTAGTATGTTCAACACACCAGGAATGCAGAGCTTGTTGC
AACAAATAACTGAAAACCCACAACTGATGCAAAACATGTTGTCTGCCCCC
TACATGAGAAGCATGATGCAGTCACTgAGCCAGAATCCTGACCTTGCTGC
ACAGATGATGCTGAATAATCCCCTATTTGCTGGAAATCCTCAGCTTCAAG
AACAAATGAGACAACAGCTCCCAACTTTCCTCCAACAAATGCAGAATCCT
GATACACTATCAGCAATGTCAAACCCTAGAGCAATGCAGGCCTTGTTACA
GATTCAGCAGGGTTTACAGACATTAGCAACGGAAGCCCCGGGCCTCATCC
CAGGGTTTACTCCTGGCTTGGGGGCATTAGGAAGCACTGGAGGCTCTTCG
GGAACTAATGGATCTAACGCCACACCTAGTGAAAACACAAGTCCCACAGC
AGGAACCACTGAACCTGGACATCAGCAGTTTATTCAGCAGATGCTGCAGG
CTCTTGCTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCAGATTTCAG
CAACAACTGGAACAACTCAaTGCAATGGGATTTTTGAACCGTGAAGCAAA
CTTGCAAGCTCTAATAGCAACAGGAGGTGATATCAATGCAGCTATTGAAA
GGTTACTGGGCTCCCAGCCATCATAGagatct
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSMAESGESGGPPGSQ
DSAAGAEGAGAPAAAASAEPKIMKVTVKTPKEKEEFAVPENSSVQQFKEE
ISKRFKSHTDQLVLIFAGKILKDQDTLSQHGIHDGLTVHLVIKTQNRPQD
HSAQQTNTAGSNVTTSSTPNSNSTSGSATSNPFGLGGLGGLAGLSSLGLN
TTNFSELQSQMQRQLLSNPEMMVQIMENPFVQSMLSNPDLMRQLIMANPQ
MQQLIQRNPEISHMLNNPDIMRQTLELARNPAMMQEMMRNQDRALSNLES
IPGGYNALRRMYTDIQEPMLSAAQEQFGGNPFASLVSNTSSGEGSQPSRT
ENRDPLPNPWAPQTSQSSSASSGTASTVGGTTGSTASGTSGQSTTAPNLV
PGVGASMFNTPGMQSLLQQITENPQLMQNMLSAPYMRSMMQSLSQNPDLA
AQMMLNNPLFAGNPQLQEQMRQQLPTFLQQMQNPDTLSAMSNPRAMQALL
QIQQGLQTLATEAPGLIPGFTPGLGALGSTGGSSGTNGSNATPSENTSPT
AGTTEPGHQQFIQQMLQALAGVNPQLQNPEVRFQQQLEQLNAMGFLNREA
NLQALIATGGDINAAIERLLGSQPS*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSMAESGESGGPPGSQ
DSAAGAEGAGAPAAAASAEPKIMKVTVKTPKEKEEFAVPENSSVQQFKEE
ISKRFKSHTDQLVLIFAGKILKDQDTLSQHGIHDGLTVHLVIKTQNRPQD
HSAQQTNTAGSNVTTSSTPNSNSTSGSATSNPFGLGGLGGLAGLSSLGLN
TTNFSELQSQMQRQLLSNPEMMVQIMENPFVQSMLSNPDLMRQLIMANPQ
MQQLIQRNPEISHMLNNPDIMRQTLELARNPAMMQEMMRNQDRALSNLES
IPGGYNALRRMYTDIQEPMLSAAQEQFGGNPFASLVSNTSSGEGSQPSRT
ENRDPLPNPWAPQTSQSSSASSGTASTVGGTTGSTASGTSGQSTTAPNLV
PGVGASMFNTPGMQSLLQQITENPQLMQNMLSAPYMRSMMQSLSQNPDLA
AQMMLNNPLFAGNPQLQEQMRQQLPTFLQQMQNPDTLSAMSNPRAMQALL
QIQQGLQTLATEAPGLIPGFTPGLGALGSTGGSSGTNGSNATPSENTSPT
AGTTEPGHQQFIQQMLQALAGVNPQLQNPEVRFQQQLEQLNAMGFLNREA
NLQALIATGGDINAAIERLLGSQPS*
Vector Type
Bacterial
Antibiotic
Amp
DU Number
DU24235
Unit Source
Location
PPU
MTA
Notes
clone harbours a1221g (silent at Leu407) compared to NCBI ref
Parental: 24192
Parental: 24192
Price
£125.00
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