cDNA Clone UBQLN1

• Synonyms: DA41, DSK2, UBQN, XDRP1, PLIC-1, FLJ90054
• Plasmid: pGEX6P-1-HALO-TEV-UBQLN1
• Parent Plasmid: pGEX6P1 Halo TEV
• Expressed: GST-HALO-UBQLN1
• Genbank: Gene 29979 Isoform 1 = NM_013438.4 (NP_038466.2)
• Tag: GST
• Tag: HALO
• Species: Human

Sequence of Insert

ggatccATGGCCGAGAGTGGTGAAAGCGGCGGTCCTCCGGGCTCCCAGGA
TAGCGCCGCCGGAGCCGAAGGTGCTGGCGCCCCCGCGGCCGCTGCCTCCG
CGGAGCCCAAAATCATGAAAGTCACCGTGAAGACCCCGAAGGAAAAGGAG
GAATTCGCCGTGCCCGAGAATAGCTCCGTCCAGCAGTTTAAGGAAGAAAT
CTCTAAACGTTTTAAATCACATACTGACCAACTTGTGTTGATATTTGCTG
GAAAAATTTTGAAAGATCAAGATACCTTGAGTCAGCATGGAATTCATGAT
GGACTTACTGTTCACCTTGTCATTAAAACACAAAACAGGCCTCAGGATCA
TTCAGCTCAGCAAACAAATACAGCTGGAAGCAATGTTACTACATCATCAA
CTCCTAATAGTAACTCTACATCTGGTTCTGCTACTAGCAACCCTTTTGGT
TTAGGTGGCCTTGGGGGACTTGCAGGTCTGAGTAGCTTGGGTTTGAATAC
TACCAACTTCTCTGAACTACAGAGTCAGATGCAGCGACAACTTTTGTCTA
ACCCTGAAATGATGGTCCAGATCATGGAAAATCCCTTTGTTCAGAGCATG
CTCTCAAATCCTGACCTGATGAGACAGTTAATTATGGCCAATCCACAAAT
GCAGCAGTTGATACAGAGAAATCCAGAAATTAGTCATATGTTGAATAATC
CAGATATAATGAGACAAACGTTGGAACTTGCCAGGAATCCAGCAATGATG
CAGGAGATGATGAGGAACCAGGACCGAGCTTTGAGCAACCTAGAAAGCAT
CCCAGGGGGATATAATGCTTTAAGGCGCATGTACACAGATATTCAGGAAC
CAATGCTGAGTGCTGCACAAGAGCAGTTTGGTGGTAATCCATTTGCTTCC
TTGGTGAGCAATACATCCTCTGGTGAAGGTAGTCAACCTTCCCGTACAGA
AAATAGAGATCCACTACCCAATCCATGGGCTCCACAGACTTCCCAGAGTT
CATCAGCTTCCAGCGGCACTGCCAGCACTGTGGGTGGCACTACTGGTAGT
ACTGCCAGTGGCACTTCTGGGCAGAGTACTACTGCGCCAAATTTGGTGCC
TGGAGTAGGAGCTAGTATGTTCAACACACCAGGAATGCAGAGCTTGTTGC
AACAAATAACTGAAAACCCACAACTGATGCAAAACATGTTGTCTGCCCCC
TACATGAGAAGCATGATGCAGTCACTgAGCCAGAATCCTGACCTTGCTGC
ACAGATGATGCTGAATAATCCCCTATTTGCTGGAAATCCTCAGCTTCAAG
AACAAATGAGACAACAGCTCCCAACTTTCCTCCAACAAATGCAGAATCCT
GATACACTATCAGCAATGTCAAACCCTAGAGCAATGCAGGCCTTGTTACA
GATTCAGCAGGGTTTACAGACATTAGCAACGGAAGCCCCGGGCCTCATCC
CAGGGTTTACTCCTGGCTTGGGGGCATTAGGAAGCACTGGAGGCTCTTCG
GGAACTAATGGATCTAACGCCACACCTAGTGAAAACACAAGTCCCACAGC
AGGAACCACTGAACCTGGACATCAGCAGTTTATTCAGCAGATGCTGCAGG
CTCTTGCTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCAGATTTCAG
CAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACCGTGAAGCAAA
CTTGCAAGCTCTAATAGCAACAGGAGGTGATATCAATGCAGCTATTGAAA
GGTTACTGGGCTCCCAGCCATCATAGagatct

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGENLYFQGGSMAESGESGGPPGS
QDSAAGAEGAGAPAAAASAEPKIMKVTVKTPKEKEEFAVPENSSVQQFKE
EISKRFKSHTDQLVLIFAGKILKDQDTLSQHGIHDGLTVHLVIKTQNRPQ
DHSAQQTNTAGSNVTTSSTPNSNSTSGSATSNPFGLGGLGGLAGLSSLGL
NTTNFSELQSQMQRQLLSNPEMMVQIMENPFVQSMLSNPDLMRQLIMANP
QMQQLIQRNPEISHMLNNPDIMRQTLELARNPAMMQEMMRNQDRALSNLE
SIPGGYNALRRMYTDIQEPMLSAAQEQFGGNPFASLVSNTSSGEGSQPSR
TENRDPLPNPWAPQTSQSSSASSGTASTVGGTTGSTASGTSGQSTTAPNL
VPGVGASMFNTPGMQSLLQQITENPQLMQNMLSAPYMRSMMQSLSQNPDL
AAQMMLNNPLFAGNPQLQEQMRQQLPTFLQQMQNPDTLSAMSNPRAMQAL
LQIQQGLQTLATEAPGLIPGFTPGLGALGSTGGSSGTNGSNATPSENTSP
TAGTTEPGHQQFIQQMLQALAGVNPQLQNPEVRFQQQLEQLSAMGFLNRE
ANLQALIATGGDINAAIERLLGSQPS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24023
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: clone harbours a1221g (silent at Leu407) compared to NCBI ref; Parental: 24005