cDNA Clone Ubiquitin

• Plasmid: pGEX6P-1-Ubiquitin monomer (Bam-Nae-Ubiquitin-Not)
• Parent Plasmid: pGEX6P1
• Genbank: P62988
• Species: Human

Sequence of Insert

GGATCCGCCGGCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACC
ATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAA
GATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTG
CTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAG
AAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGCGGCTGATAAGC
GGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAGMQIFVKTLTGKTITLEV
EPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTL
HLVLRLRGG**

• Vector Type: Bacterial
• Antibiotic: Amp; Kan
• DU Number: DU20709
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: GST-ubiquitin (wild type) monomer and intermediate in the assembly of wild type polyubiquitin chains; ; Estimated MW of expressed protein : 35517 Daltons