cDNA Clone USP49

• Plasmid: pGEX6P-USP49 L240-end (cat domain)
• Parent Plasmid: pGEX6P3
• Expressed: GST-USP49 240-end (cat domain)
• Genbank: NM_018561.3 GI:34147686
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTCTCAAGCTGCGTCGCCAGCCGGCCATGGCCCCAGGCGTCACGGG
CCTGCGCAACCTGGGCAACACCTGCTACATGAACTCCATCCTCCAGGTGC
TCAGCCACCTCCAGAAGTTCCGAGAATGTTTCCTCAACCTTGACCCTTCC
AAAACGGAACATCTGTTTCCCAAAGCCACCAACGGGAAGACTCAGCTTTC
TGGCAAGCCAACCAACAGCTCGGCCACGGAGCTGTCCTTGAGAAATGACA
GGGCCGAGGCATGCGAGCGGGAGGGCTTCTGCTGGAACGGCAGGGCCTCC
ATTAGTCGGAGTCTGGAGCTCATCCAGAACAAGGAGCCGAGTTCAAAGCA
CATTTCCCTCTGCCGTGAACTGCACACCCTCTTCCGAGTCATGTGGTCCG
GGAAGTGGGCCCTAGTGTCGCCCTTCGCCATGCTGCACTCAGTGTGGAGC
CTGATCCCTGCCTTCCGCGGCTACGACCAACAGGACGCGCAGGAATTTCT
CTGCGAGCTGCTGCACAAGGTGCAGCAGGAACTCGAGTCTGAGGGCACCA
CACGCCGGATCCTCATCCCCTTCTCCCAGAGGAAGCTCACCAAACAGGTC
TTAAAGGTGGTGAATACCATATTTCATGGGCAGCTGCTCAGTCAGGTCAC
ATGTATATCATGCAATTACAAATCCAATACCATTGAGCCCTTTTGGGACC
TATCCCTGGAATTCCCTGAACGCTATCACTGCATAGAAAAGGGGTTTGTC
CCTTTGAATCAAACAGAGTGCTTGCTCACTGAGATGCTGGCCAAATTCAC
AGAGACAGAGGCCCTGGAAGGGAGAATCTACGCTTGTGACCAGTGTAACA
GCAAACGACGAAAATCCAATCCCAAACCCCTTGTTCTGAGTGAAGCTAGA
AAGCAGTTAATGATCTACAGACTACCTCAGGTTCTCCGGCTGCACCTTAA
AAGATTCAGGTGGTCTGGCCGTAATCATCGAGAGAAGATTGGGGTCCATG
TCGTCTTTGACCAGGTATTAACCATGGAACCTTACTGCTGCAGGGACATG
CTCTCCTCTCTTGACAAAGAGACCTTTGCCTATGATCTCTCCGCAGTGGT
CATGCATCACGGGAAAGGGTTTGGCTCAGGACACTACACAGCCTATTGCT
ACAACACAGAGGGAGGTGCGTGCGCTTTACTCTGTGGGGTGGGGGACACG
GAAAGGGGTTGAAGATCT

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSLKLRRQPAMAPGVTGLRNL
GNTCYMNSILQVLSHLQKFRECFLNLDPSKTEHLFPKATNGKTQLSGKPT
NSSATELSLRNDRAEACEREGFCWNGRASISRSLELIQNKEPSSKHISLC
RELHTLFRVMWSGKWALVSPFAMLHSVWSLIPAFRGYDQQDAQEFLCELL
HKVQQELESEGTTRRILIPFSQRKLTKQVLKVVNTIFHGQLLSQVTCISC
NYKSNTIEPFWDLSLEFPERYHCIEKGFVPLNQTECLLTEMLAKFTETEA
LEGRIYACDQCNSKRRKSNPKPLVLSEARKQLMIYRLPQVLRLHLKRFRW
SGRNHREKIGVHVVFDQVLTMEPYCCRDMLSSLDKETFAYDLSAVVMHHG
KGFGSGHYTAYCYNTEGGACALLCGVGDTERG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36250
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 72783 Daltons