Full Name
IRAK1 (mouse)
Long Name Textual
Interleukin-1 Receptor-Associated Kinase
Immuno Sequence
GST cleaved IRAK1 mouse [DU 32043]
Mono/Poly clonal
Polyclonal
Gel Image
Raw cell lysates (1mg protein) were immunoprecipitated with increasing amounts of mouse anti-IRAK1 antibody or pre-immune serum for 2hr along with 10 μl of protein G. Immunoprecipitated proteins were denatured in SDS and subjected to 8% SDS-PAGE (Lanes 1-4). Lanes 5- 8 indicate the amount of mouse IRAK1 protein remaining in the supernatant after immunoprecipitation, the amounts loaded being equivalent to 25 μg of extract protein. Proteins were transferred to PVDF membrane and immunoblotting was carried out using rabbit polyclonal antibody against IRAK1 (Santa Cruz) overnight at 1:1000.
Sheep No
S690C
Unit Source
Aliquot
0.1mg
Price
£150.00