Full Name
NCC phospho Thr 60
Immuno Sequence
RTFGYNT*IDVVPTRR [residues 54 - 66 of human + two C-terminal arginine residues]
Mono/Poly clonal
Polyclonal
Gel Image
HEK 293 cells were transfected with wildtype human NCC. 36 hours post-transfection, cells were treated with either basic or hyptonic low chloride medium for the specified time-points and lysed. The cell lysates were subjected to SDS-Page, followed by immunoblotting with anti-NCC phospho Thr 46 [S241C], anti-NCC phospho Thr 55 [S908B], anti-NCC phospho Thr 60 [S995B], anti-NCC phospho Ser 91 [S996B] and anti-NCC (AB3553 Chemicon) antibodies. Binding of the phospho specific primary antibodies was detected using rabbit peroxidase conjugated anti-sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).
Sheep No
S995B
Unit Source
Aliquot
0.1mg
Price
£150.00