cDNA Clone Ubiquitin

• Synonyms: cLK58
• Plasmid: pET24a M1-Ub2 [wt, 1-72 K33A all other K-to-R] (cLK58)
• Parent Plasmid: pET24a
• Expressed: M1-Ub2 [wt, 1-72 K33A all other K-to-R]
• Genbank: NM_021009.7
• Species: Human

Amino Acid Sequence

MQIFVRTLTGRTITLEVEPSDTIENVRARIQDAEGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLVLR

• Vector Type: Bacterial
• Full Snapgene Map: pET24a M1-Ub2 [wt, 1-72 K33A all other K-to-R] (cLK58)719.92 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU75887
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: QuickChange with KOD Hot Start Polymerase. Diubiquitin. First copy is WT M1-G76, second copy is M1- R72 all K/R except K33A. Plasmid deposited by Logesvaran krshnan (Yogesh Kulathu group MRCPPU)
• Low Temp: No