cDNA Clone Ufl1

• Plasmid: petDUet1 6His TEV Ufl1 (1-410) C32, C143,C300,C372A/StrepII 3C DDRGK1 A29-end
• Parent Plasmid: pETDuet1
• Expressed: His6-TEV-Ufl1(M1-V410) C32, C143,C300,C372A
• Genbank: NM_015323.5
• Tag: 6His
• Species: Human

Sequence of Insert

ccATGGGACACCATCACCATCACCATGAAAACCTGTATTTTCAGATGGCGGACGCCTGGGAAGAGATTAGGCGGTTGGCGGCCGACTTCCAGCGGGCGCAGTTCGCCGAGGCCACGCAGAGGTTGTCCGAGCGGAACGCCATTGAGATTGTTAATAAATTGATTGCTCAGAAACAGCTAGAAGTAGTTCATACACTCGATGGAAAGGAATATATTACTCCAGCCCAAATTAGTAAAGAAATGAGAGATGAGCTACATGTCCGAGGTGGTCGAGTAAACATTGTTGATCTACAACAGGTAATTAATGTGGACCTGATTCATATTGAAAATAGAATTGGTGACATTATTAAATCAGAAAAGCATGTTCAGTTAGTGTTGGGACAACTGATAGATGAGAATTATTTGGATCGGTTGGCAGAAGAGGTCAATGATAAATTGCAAGAAAGTGGTCAGGTCACCATATCAGAACTGGCTAAAACTTATGATCTTCCTGGGAACTTTCTGACACAGGCACTAACTCAGCGACTTGGTAGAATTATCAGTGGACATATTGATCTTGATAATAGAGGAGTAATTTTTACGGAAGCTTTTGTAGCTCGACATAAAGCACGTATCCGTGGACTATTCAGTGCTATTACCCGGCCTACAGCTGTGAATTCTTTGATTTCAAAATATGGATTTCAGGAGCAGCTTCTTTACTCTGTGCTTGAGGAACTTGTTAATAGCGGACGCTTACGAGGCACTGTGGTTGGTGGGAGACAGGATAAAGCTGTGTTTGTCCCTGACATCTACTCCAGGACACAGAGTACTTGGGTGGATTCCTTTTTCAGGCAGAATGGCTATCTAGAATTTGATGCTTTGTCCAGACTTGGAATCCCAGATGCTGTAAGCTACATAAAGAAAAGATATAAGACTACACAACTCTTGTTTTTGAAAGCAGCTGCTGTTGGTCAAGGACTTGTGGATCAAGTGGAAGCATCAGTAGAAGAAGCCATCAGCTCTGGAACATGGGTTGATATTGCACCTCTGCTACCCACTTCTTTATCAGTTGAAGATGCTGCCATATTGCTTCAGCAGGTGATGAGGGCATTCAGCAAACAGGCCTCAACTGTAGTCTTTAGCGACACTGTTGTAGTCAGTGAAAAATTTATAAATGACGCTACAGAACTGTTCCGTGAGCTGATGCACCAGAAAGCTGAAAAGGAAATGAAAAATAATCCTGTGCATTTAATCACTGAAGAAGATCTGAAACAAATCTCCACTTTAGAAAGCGTTTAAgcggccgc

Amino Acid Sequence

MGHHHHHHENLYFQMADAWEEIRRLAADFQRAQFAEATQRLSERNAIEIVNKLIAQKQLEVVHTLDGKEYITPAQISKEMRDELHVRGGRVNIVDLQQVINVDLIHIENRIGDIIKSEKHVQLVLGQLIDENYLDRLAEEVNDKLQESGQVTISELAKTYDLPGNFLTQALTQRLGRIISGHIDLDNRGVIFTEAFVARHKARIRGLFSAITRPTAVNSLISKYGFQEQLLYSVLEELVNSGRLRGTVVGGRQDKAVFVPDIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDAVSYIKKRYKTTQLLFLKAAAVGQGLVDQVEASVEEAISSGTWVDIAPLLPTSLSVEDAAILLQQVMRAFSKQASTVVFSDTVVVSEKFINDATELFRELMHQKAEKEMKNNPVHLITEEDLKQISTLESV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU66290
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Kindly mutate C32, C143,C300,C372 to Alanine. Please use Gene synthesis. Also, I would like to co express this with StrepII tagged DDRGK1 as in DU66224.
• Low Temp: No